Algae & Bacteria > Chlorophyll Fluorescence > Z985 Cuvette AquaPen

Z985 Cuvette AquaPen

Starting from $2,900

Z985 Cuvette AquaPen is a cuvette version of the AquaPen fluorometer. It has blue and red measuring lights that enable measurements of photosynthetic parameters both in algal and cyanobacterial suspensions in a cuvette.  Due to its ultra-high sensitivity – up to 0.5 μg Chl/L, the Cuvette AquaPen can make measurements in natural water samples containing low concentrations of phytoplankton.

The Z985 Cuvette AquaPen includes the protocol update and software plus data transfer option with bluetooth, USB or serial communication technology.  A Probe (Z980) version of the AquaPen allows the same measurements of chlorophyll fluorescence in suspension by directly placing the probe in the suspension medium.

Applications:

  • Photosynthesis research of algal and cyanobacterial suspensions
  • Photosynthesis education
  • Phycology
  • Limnology
  • Oceanography
  • Biotechnology

measured/calculated parameters:

  • FT: Instantaneous chlorophyll fluorescence. Ft is equivalent to Fo if the sample is dark-adapted.
  • QY: Quantum Yield. QY is a measure of the Photosystem II efficiency. QY is equivalent to Fv/Fm in dark-adapted samples and to Fv’/Fm’ in light-adapted samples.
  • OJIP: Chlorophyll fluorescence transient. OJIP measurement is used as an important biophysical signal that reflects the time course of photosynthesis
  • NPQ: Non-photochemical quenching. NPQ indicates thermal dissipation of absorbed light energy during photosynthesis.
  • LC: Light Curve. LC1 and LC2 protocols serve to describe adaptation of QY to 5 or 6 light levels.
  • Optical Density : by two IR LEDs (735 nm, 680 nm)

 

Software:

  • FluorPen 1.0 software (Windows 2000, XP, or higher compatible*)
  • Bluetooth, USB or serial communication
  • Real-time and remote control functions
  • Export to Microsoft Excel
  • GPS mapping plug-in (optional)

NPQ Protocol Includes Five Measurements in Actinic Light
and Three Measurements During Dark Relaxation.
NPQ_Ln = (FM – FM_Ln) / FM_Ln
NPQ_Lss = (FM – FM_Lss) / FM_Lss
NPQ_Dn = (FM – FM_Dn) / FM_Dn

OJIP cuve with data sheet

Explanation of OJIP Parameters:

  • Bckg = background
  • F0: = F50µs; fluorescence intensity at 50 µs
  • FJ: = fluorescence intensity at j-step (at 2 ms)
  • Fi: = fluorescence intensity at i-step (at 60 ms)
  • FM: = maximal fluorescence intensity
  • FV: = FM – F0 (maximal variable fluorescence)
  • VJ = (FJ – F0) / (FM – F0)
  • Vi = (Fi – F0) / (FM – F0)
  • FM / F0
  • FV / F0
  • FV/ FM
  • M0 or (dV / dt)0 = TR0 / RC – ET0 / RC = 4 (F300 – F0) / (FM – F0)
  • Area = area between fluorescence curve and FM (background subtracted)
  • Fix Area = total area above the OJIP fluorescence transient – between F40µ and F1s(background subtracted)
  • SM = area / FM – F0 (multiple turn-over)
  • Ss = the smallest Sm turn-over (single turn-over)
  • N = SM . M0 . (1 / VJ) turn-over number QA
  • Phi_P0 = 1 – (F0 / FM (or FV / FM)
  • Psi_0 = 1 – VJ
  • Phi_E0 = (1 – F0 / FM)) . Psi_0
  • Phi_D0 = 1 – Phi_P0 – (F0 / FM)
  • Phi_Pav = Phi_P0 – (SM / tFM); tFM) = Time to reach FM (in ms)
  • ABS / RC = M0 . (1 / VJ) . (1 / Phi_P0)
  • TR0 / RC = M0 . (1 / VJ)
  • ET0 / RC = M0 . (1 / VJ) . Phi_0)
  • DI0 / RC = (ABS / RC) – (TR0 / RC)

Formulas Derived From:
R.J. Strasser, A. Srivastava and M. Tsimilli-Michael (2000): The fluorescence transient as a tool to characterize and screen photosynthetic samples. In: Probing Photosynthesis: Mechanism, Regulation and Adaptation (M. Yunus, U. Pathre and P. Mohanty, eds.), Taylor and Francis, UK, Chapter 25, pp 445-483.

Light Curve data

Specifications:

  • Measured/Calculated Parameters:F0 , FT , FM , FM ‘ , QY, OJIP, NPQ, LC 1, LC 2, OD 680 , OD 720
  • Actinic and Saturating Light: Adjustable from 0 to 3,000 µmol (photons).m-2.s-1
  • Measuring Light: Red and blue measuring lights adjustable by intensity
  • Detector Wavelength Range: PIN photodiode with 667 to 750 nm bandpass filters
  • FluorPen 1.0 Software: for Windows 2000, XP, or higher
  • Memory Capacity: Up to 4 Mb
  • Internal Data Logging: Up to 100,000 data points
  • Display: 2 x 8 characters LC display
  • Keypad: Sealed, 2-key tactile response
  • Keypad Escape Time: Turns off after 3 minutes of no use
  • Power Supply: 4 AAA alkaline or rechargeable batteries
  • Battery Life: 48 hours typical with full operation
  • Low Battery Detection: Low battery indication displayed
  • Size: 140 x 55 x 30 mm
  • Weight: 300 g, 10.6 oz
  • Sample Holder: 4 ml cuvette
  • Operating Conditions: Temperature: 0 to 55 ºC; 32 to 130 ºF. Relative humidity: 0 to 95 % (non-condensing)
  • Storage Conditions: Temperature: -10 to +60 ºC; 14 to +140 ºF. Relative humidity: 0 to 95 % (non-condensing)
  • Warranty: 1 year parts and labor

References:

  • Piovar J. Stavrou E, Kaducova J, Kimakova T, Backor M.  Influence of long-term exposure to copper on the lichen photobiont Trebouxia erici and the free-living algae Scenedesmus quadricauda: Plant Growth Regulation (2010) doi:10.1007/s10725-010-9515-4
  • Vandamme D.  Foubert I, Meesschaert B, Muylaert K.   Flocculation of microalgae using cationic starch: Journal of Applied Phycology (2010) 22:525-530
  • Gao Q.T. and N.F.Y. Tam. Growth, photosynthesis and antioxidant responses of two microalgal species, Chlorella vulgaris and Selenastrum capricornutum, to nonylphenol stress: Chemosphere (2010) doi:10.1016
  • Kvíderová J.  Rapid algal toxicity assay using variable chlorophyll fluorescence for Chlorella kessleri (Chlorophyta): Environmental Toxicology (2010) doi:10.1002