is used to monitor and regulate pH or pCO2 in sea and fresh water in up to 4 different tanks!This automated feedback system will determine pCO2 indirectly as water pH, and regulate pCO2 or pH to a level set by the user. The user-friendly windows software Cap CTRL makes it possible to monitor calibrated values for pH and pCO2 in a real-time semi log plot (log pCO2 vs. pH), and control either to set point levels automatically.
With optional accessories this system will also regulate water temperature. Applications range from studies of ocean acidification, water chemistry, effects of hypercapnia in aquatic organisms, and normocapnic control in recirculated or closed tanks and aquaria.
- WTW pH 3310 SET incl. SenTix pH probe for fresh or sea water
- Buffers, suitcase and USB cable
- Digital relay instrument with USB interface (DAQ-M)
- Cap CTRL software
- Solenoid valve
- Push-in fittings
- PU air tubing
- Air stone
- User manual
- galvanic isolator if more than one pH probe used
TYPICAL EXPERIMENTAL SETUP
- pCO2 is monitored indirectly as water pH, and regulated by injection of 100% CO2 gas or air
- A pH 3310 instrument and probe from German WTW is connected to your PC via USB
- User-friendly Cap CTRL software for Windows, allows you to calibrate, graph, record and regulate water pH or pCO2 values to pre set values, e.g. for normocapnic or hypercapnic control
- USB instrument with build-in digital relays, complete with a solenoid valve and fittings, will control injection of CO2 gas or other gasses
- The relationship between pCO2 and pH is obtained by measuring pH in water equilibrated with two different pCO2 partial pressures, e.g. use a gas mixing pump (Woesthoff) or special lab gases
WTW pH 3310: pCO2 calibration:
- SenTix H pH electrode + cable for measurements in sea water.
- D203 for water temperature regulation.
- One extra D202 and one extra WTW pH 3310 SET for independent pH/pCO2 regulation in one more tank/aquarium.
The following references used the same technique to control pCO2.
D.J. McKenzie, A.Z. Dalla Valle, M. Piccolella, E.W. Taylor and J.F. Steffensen. 2003. Tolerance of chronic hypercapnia by the European eel (Anguilla anguilla). J. exp. Biol. 206: 1717-1726.
D.J. McKenzie, E.W. Taylor, A.Z. Dalla Valle and J.F. Steffensen. 2002. Tolerance of acute hypercapnic acidosis by the European eel (Anguilla anguilla). J. Comp. Physiol. B 172: 347-354.
Cruz-Neto, A. and Steffensen, J. F. 1997. The effects of acute hypoxia and hypercapnia oxygen consumption of freshwater European eel, Anguilla anguilla L. J. Fish Biol. 50; 759-769.