Qubit Systems Inc. » Teaching

Z985 Cuvette AquaPen

admin — Sat, 12 Mar 2011 13:44:43 +0000

Z985 Cuvette AquaPen is a cuvette version of the AquaPen fluorometer. It has blue and red measuring lights that enable measurements of photosynthetic parameters both in algal and cyanobacterial suspensions in a cuvette. Due to its ultra-high sensitivity – up to 0.5 μg Chl/L, the Cuvette AquaPen can make measurements in natural water samples containing low concentrations of phytoplankton.

The Z985 Cuvette AquaPen includes the protocol update and software plus data transfer option with bluetooth, USB or serial communication technology. A Probe (Z980) version of the AquaPen allows the same measurements of chlorophyll fluorescence in suspension by directly placing the probe in the suspension medium.

Applications:

Photosynthesis research of algal and cyanobacterial suspensions
Photosynthesis education
Phycology
Limnology
Oceanography
Biotechnology

measured/calculated parameters:

FT: Instantaneous chlorophyll fluorescence. Ft is equivalent to Fo if the sample is dark-adapted.
QY: Quantum Yield. QY is a measure of the Photosystem II efficiency. QY is equivalent to Fv/Fm in dark-adapted samples and to Fv’/Fm’ in light-adapted samples.
OJIP: Chlorophyll fluorescence transient. OJIP measurement is used as an important biophysical signal that reflects the time course of photosynthesis
NPQ: Non-photochemical quenching. NPQ indicates thermal dissipation of absorbed light energy during photosynthesis.
LC: Light Curve. LC1 and LC2 protocols serve to describe adaptation of QY to 5 or 6 light levels.
Optical Density : by two IR LEDs (735 nm, 680 nm)

Software:

FluorPen 1.0 software (Windows 2000, XP, or higher compatible*)
Bluetooth, USB or serial communication
Real-time and remote control functions
Export to Microsoft Excel
GPS mapping plug-in (optional)

NPQ Protocol Includes Five Measurements in Actinic Light
and Three Measurements During Dark Relaxation.
NPQ_Ln = (FM – FM_Ln) / FM_Ln
NPQ_Lss = (FM – FM_Lss) / FM_Lss
NPQ_Dn = (FM – FM_Dn) / FM_Dn

OJIP cuve with data sheet

Explanation of OJIP Parameters:

Bckg = background
F0: = F50µs; fluorescence intensity at 50 µs
FJ: = fluorescence intensity at j-step (at 2 ms)
Fi: = fluorescence intensity at i-step (at 60 ms)
FM: = maximal fluorescence intensity
FV: = FM – F0 (maximal variable fluorescence)
VJ = (FJ – F0) / (FM – F0)
Vi = (Fi – F0) / (FM – F0)
FM / F0
FV / F0
FV/ FM
M0 or (dV / dt)0 = TR0 / RC – ET0 / RC = 4 (F300 – F0) / (FM – F0)
Area = area between fluorescence curve and FM (background subtracted)
Fix Area = total area above the OJIP fluorescence transient – between F40µ and F1s(background subtracted)
SM = area / FM – F0 (multiple turn-over)
Ss = the smallest Sm turn-over (single turn-over)
N = SM . M0 . (1 / VJ) turn-over number QA
Phi_P0 = 1 – (F0 / FM (or FV / FM)
Psi_0 = 1 – VJ
Phi_E0 = (1 – F0 / FM)) . Psi_0
Phi_D0 = 1 – Phi_P0 – (F0 / FM)
Phi_Pav = Phi_P0 – (SM / tFM); tFM) = Time to reach FM (in ms)
ABS / RC = M0 . (1 / VJ) . (1 / Phi_P0)
TR0 / RC = M0 . (1 / VJ)
ET0 / RC = M0 . (1 / VJ) . Phi_0)
DI0 / RC = (ABS / RC) – (TR0 / RC)

Formulas Derived From:
R.J. Strasser, A. Srivastava and M. Tsimilli-Michael (2000): The fluorescence transient as a tool to characterize and screen photosynthetic samples. In: Probing Photosynthesis: Mechanism, Regulation and Adaptation (M. Yunus, U. Pathre and P. Mohanty, eds.), Taylor and Francis, UK, Chapter 25, pp 445-483.

Light Curve data

Specifications:

Measured/Calculated Parameters:F0 , FT , FM , FM ‘ , QY, OJIP, NPQ, LC 1, LC 2, OD 680 , OD 720
Actinic and Saturating Light: Adjustable from 0 to 3,000 µmol (photons).m-2.s-1
Measuring Light: Red and blue measuring lights adjustable by intensity
Detector Wavelength Range: PIN photodiode with 667 to 750 nm bandpass filters
FluorPen 1.0 Software: for Windows 2000, XP, or higher
Memory Capacity: Up to 4 Mb
Internal Data Logging: Up to 100,000 data points
Display: 2 x 8 characters LC display
Keypad: Sealed, 2-key tactile response
Keypad Escape Time: Turns off after 3 minutes of no use
Power Supply: 4 AAA alkaline or rechargeable batteries
Battery Life: 48 hours typical with full operation
Low Battery Detection: Low battery indication displayed
Size: 140 x 55 x 30 mm
Weight: 300 g, 10.6 oz
Sample Holder: 4 ml cuvette
Operating Conditions: Temperature: 0 to 55 ºC; 32 to 130 ºF. Relative humidity: 0 to 95 % (non-condensing)
Storage Conditions: Temperature: -10 to +60 ºC; 14 to +140 ºF. Relative humidity: 0 to 95 % (non-condensing)
Warranty: 1 year parts and labor

References:

Piovar J. Stavrou E, Kaducova J, Kimakova T, Backor M. Influence of long-term exposure to copper on the lichen photobiont Trebouxia erici and the free-living algae Scenedesmus quadricauda: Plant Growth Regulation (2010) doi:10.1007/s10725-010-9515-4
Vandamme D. Foubert I, Meesschaert B, Muylaert K. Flocculation of microalgae using cationic starch: Journal of Applied Phycology (2010) 22:525-530
Gao Q.T. and N.F.Y. Tam. Growth, photosynthesis and antioxidant responses of two microalgal species, Chlorella vulgaris and Selenastrum capricornutum, to nonylphenol stress: Chemosphere (2010) doi:10.1016
Kvíderová J. Rapid algal toxicity assay using variable chlorophyll fluorescence for Chlorella kessleri (Chlorophyta): Environmental Toxicology (2010) doi:10.1002

Z980 Probe AquaPen

admin — Fri, 11 Mar 2011 12:55:56 +0000

The Z980 Probe AquaPen is a handheld fluorometer that enables rapid and accurate measurement of photosynthetic parameters in suspensions. It is equipped with a blue measuring light that suits investigations in algal suspensions. The measurements are carried out by a submersible optical probe. The AquaPen excels in a very high sensitivity (up to 0.5 μg Chl/L) and it can even be used in measurements in natural water samples containing low concentrations of phytoplankton. It can be used both in the lab and field applications or for teaching.

The Z980 Probe AquaPen includes the Protocol Update and Software, plus Data Transfer option with Bluetooth, USB or serial communication technology. A cuvette (Z985) version of the AquaPen allows the same chlorophyll fluorescence measurements in suspension contained in a 4 ml cuvette

Applications:

Photosynthesis Research of algae and cyanobacteria
Photosynthesis Education
Phycology
Limnology
Oceanography
Biotechnology

measured/calculated parameters:

FT: Instantaneous chlorophyll fluorescence. Ft is equivalent to Fo if the sample is dark-adapted.
QY: Quantum Yield. QY is a measure of the Photosystem II efficiency. QY is equivalent to Fv/Fm in dark-adapted samples and to Fv’/Fm’ in light-adapted samples.
OJIP: Chlorophyll fluorescence transient. OJIP measurement is used as an important biophysical signal that reflects the time course of photosynthesis
NPQ: Non-photochemical quenching. NPQ indicates thermal dissipation of absorbed light energy during photosynthesis.
LC: Light Curve. LC1 and LC2 protocols serve to describe adaptation of QY to 5 or 6 light levels.

Software:

FluorPen 1.0 software (Windows 2000, XP, or higher compatible*)
Bluetooth, USB or serial communication
Real-time and remote control functions
Export to Microsoft Excel
GPS mapping plug-in (optional)

OJIP cuve with data sheet

Explanation of OJIP Parameters:

Bckg = background
F0: = F50µs; fluorescence intensity at 50 µs
FJ: = fluorescence intensity at j-step (at 2 ms)
Fi: = fluorescence intensity at i-step (at 60 ms)
FM: = maximal fluorescence intensity
FV: = FM – F0 (maximal variable fluorescence)
VJ = (FJ – F0) / (FM – F0)
Vi = (Fi – F0) / (FM – F0)
FM / F0
FV / F0
FV/ FM
M0 or (dV / dt)0 = TR0 / RC – ET0 / RC = 4 (F300 – F0) / (FM – F0)
Area = area between fluorescence curve and FM (background subtracted)
Fix Area = total area above the OJIP fluorescence transient – between F40µ and F1s(background subtracted)
SM = area / FM – F0 (multiple turn-over)
Ss = the smallest Sm turn-over (single turn-over)
N = SM . M0 . (1 / VJ) turn-over number QA
Phi_P0 = 1 – (F0 / FM (or FV / FM)
Psi_0 = 1 – VJ
Phi_E0 = (1 – F0 / FM)) . Psi_0
Phi_D0 = 1 – Phi_P0 – (F0 / FM)
Phi_Pav = Phi_P0 – (SM / tFM); tFM) = Time to reach FM (in ms)
ABS / RC = M0 . (1 / VJ) . (1 / Phi_P0)
TR0 / RC = M0 . (1 / VJ)
ET0 / RC = M0 . (1 / VJ) . Phi_0)
DI0 / RC = (ABS / RC) – (TR0 / RC)

Light Curve data

Specifications:

Measured/Calculated Parameters: F0, FT, FM, FM’ , QY, OJIP, NPQ, LC 1, LC 2,
Actinic and Saturating Light: Adjustable from 0 to 3,000 µmol (photons) m-2 s-1
Measuring Light: Blue measuring light adjustable by intensity
Detector Wavelength Range: PIN photodiode with 667 to 750 nm bandpass filters
FluorPen 1.0 Software for Windows 2000, XP, or higher
Memory Capacity: Up to 4 Mb
Internal Data Logging: Up to 100,000 data points
Display: 2 x 8 characters LC display
Keypad: Sealed, 2-key tactile response
Keypad Escape Time: Turns off after 3 minutes of no use
Power Supply: 4 AAA alkaline or rechargeable batteries
Battery Life: 48 hours typical with full operation
Low Battery Detection: Low battery indication displayed
Size: 210 x 55 x 30 mm (including the probe)
Weight: 300 g (10.6 oz)
Sample Holder: Submersible optical probe
Operating Conditions: Temperature: 0 to +55ºC; 32 to +130ºF, Relative humidity: 0 to 95% (non-condensing)
Storage Conditions: Temperature: -10 to +60ºC; 14 to +140ºF, Relative humidity: 0 to 95% (non-condensing)
Warranty: 1 year parts and labor

References:

Piovar J. Stavrou E, Kaducova J, Kimakova T, Backor M. Influence of long-term exposure to copper on the lichen photobiont Trebouxia erici and the free-living algae Scenedesmus quadricauda: Plant Growth Regulation (2010) doi:10.1007/s10725-010-9515-4
Vandamme D. Foubert I, Meesschaert B, Muylaert K. Flocculation of microalgae using cationic starch: Journal of Applied Phycology (2010) 22:525-530
Gao Q.T. and N.F.Y. Tam. Growth, photosynthesis and antioxidant responses of two microalgal species, Chlorella vulgaris and Selenastrum capricornutum, to nonylphenol stress: Chemosphere (2010) doi:10.1016
Kvíderová J. Rapid algal toxicity assay using variable chlorophyll fluorescence for Chlorella kessleri (Chlorophyta): Environmental Toxicology (2010) doi:10.1002

OX1LP-50mL Dissolved O2 Package

admin — Wed, 14 Apr 2010 17:40:05 +0000

The OX1LP-50mL Dissolved O2 Package may be used to measure photosynthesis and/or respiration in the aqueous phase, using suspensions of plants, animals and organelles (chloroplasts and mitochondria). It may also be used to monitor chemical and biochemical reactions that either produce or consume O2 in the aqueous phase. The Dissolved Oyxgen Package comes complete with instruments, software and interface.

The OX1LP-50mL Dissolved Oxygen Package includes:

G107 Dissolved Oxygen Cuvette Electrode (50 mL)
A231 Cuvette Electrode Amplifier
A255 Magnetic Stirrer
A135 Stir Bar (2) and O-Ring (2)
A131 KCl Filling Solution
A136 Membrane Material and Filter Paper
10 ft Excelon Tubing for the Water Jacket
C410 LabPro Interface
C901 Logger Pro Software
C404 Customized Setup Software
Instructor’s and Student’s Manuals

The OX1LP-50mL Dissolved Oxygen Package has features included in no other polarographic O2 monitoring system. The O2 electrode within the water-jacketed cuvette may be used for measurements of O2 consumption (or production) by biological samples or enzymatic reactions. Calibration is a simple 2-point process, and all the requirements for calibration are supplied as part of the package.

For many O2 electrodes, changing the membrane is a tedious and awkward process. Not so with Qubit Systems’s O2 electrode. The membrane is located under the cuvette, which screws onto the base plate, and requires only a few seconds to change. All materials for replacing the membrane (filter paper and membrane material) are included in the Dissolved Oxygen Package.

Using C901 Logger Pro software, the O2 electrode may be calibrated in units of mg O2/L, % dissolved O2, ppm, or whatever units are appropriate for your study. The Dissolved Oxygen Package provides excellent accuracy and a resolution of 0.1% O2 (where air saturated water = 100%). Dissolved O2 values may be expressed in any desired units (mg/L; % dissolved O2; ppm etc) based on a simple calibration procedure. The water jacket allows calibrations and experiments to be carried out at the same temperature, or a simple temperature correction can be made in software for measurements made at different temperatures.

Applications

The Dissolved Oxygen Package may be used for any application that involves measurement of O2 in aqueous solutions. It can be used in the laboratory for measurement of O2 concentrations from samples taken from rivers and lakes, or to measure O2 consumption (or production) by living organisms. The Dissolved O2 Package is a perfect complement to Qubit Systems’ Respiration, Plant CO2 Analysis and Photosynthesis Packages. While these packages are designed to measure the exchange of respiratory and photosynthetic gases in the gas phase, the O2 electrode allows similar measurements to be made on aquatic organisms, and also on isolated organelles, such as mitochondria and chloroplasts.

Experiments Include:

Measurement of Aquatic Photosynthetic O2 Evolution
Effect of Inhibitors on Mitochondrial Electron Transport
Analysis of Light-dependent Chloroplast Electron Transport
Effects of Temperature on O2 Uptake by Aquatic Animals
Kinetics of O2-consuming Enzymes

Optional Components:

Syringes for addition of metabolites to cuvette; (A141) 10µL, (A143) 50µL, (A145) 100µL
S172 Thermistor to measure cuvette sample temperature
A111 Variable halogen light source
S141 Light sensor

Experimental data showing the oxygen consumption rate of Glucose Oxidase using the OX1LP Dissolved Oxygen Package:

Other OX1LP Packages:

References:

Iris Bauer and Andreas Kappler. Rates and Extent of Reduction of Fe(III) Compounds and O₂ by Humic Substances. Environ. Sci. Technol. Vol 43, Number 13, p4902–4908 ( 2009).

Carrie M. Mosher, Matthew A. Hummel, Timothy S. Tracy and Allan E. Rettie. Functional Analysis of Phenylalanine Residues in the Active Site of Cytochrome P450 2C9. Biochemistry Vol 47, Number 45, p11725–11734 (2008).

Bryce A. Mendelsohn, Bethany L. Kassebaum and Jonathan D. Gitlin. The zebrafish embryo as a dynamic model of anoxia tolerance. Developmental Dynamics Vol 237, Issue 7, p1780–1788 (2008).

Bryce A. Mendelsohn and Jonathan D. Gitlin. Coordination of development and metabolism in the pre-midblastula transition zebrafish embryo. Developmental Dynamics Vol 237, Issue 7, p1789–1798 (2008).

Johnson EA, Rosenberg J, McCarty RE. Expression by Chlamydomonas reinhardtii of a chloroplast ATP synthease with polyhistidine-tagged beta subunits. Biochimica et Biophysica Acta 1767:374-380 (2007)

Johnson E A. Altered expression of the chloroplasts ATP synthase through site-directed mutagenesis in Chlamydamonas Reinhardtii. Photosynth Res vol 96:153-162 (2008)

Charles W. Locuson, Peter M. Gannett and Timothy S. Tracy. Heteroactivator effects on the coupling and spin state equilibrium of CYP2C9. Archives of Biochemistry and Biophysics Vol 449, Issues 1-2, p115-129 (2006).

OX1LP-30mL Dissolved O2 Package

admin — Wed, 14 Apr 2010 17:17:08 +0000

The OX1LP-30mL Dissolved O2 Package may be used to measure photosynthesis and/or respiration in the aqueous phase, using suspensions of plants, animals and organelles (chloroplasts and mitochondria). It may also be used to monitor chemical and biochemical reactions that either produce or consume O2 in the aqueous phase. The Dissolved Oxygen Package comes complete with instruments, software and interface.

The OX1LP-30mL Dissolved Oxygen Package includes:

G109 Dissolved Oxygen Cuvette Electrode (30 mL)
A231 Cuvette Electrode Amplifier
A255 Magnetic Stirrer
A135 Stir Bar (2) and O-Ring (2)
A131 KCl Filling Solution
A136 Membrane Material and Filter Paper
10 ft Excelon Tubing for the Water Jacket
C410 LabPro Interface
C901 Logger Pro Software
C404 Customized Setup Software
Instructor’s and Student’s Manuals

The OX1LP-30mL Dissolved Oxygen Package has features included in no other polarographic O2 monitoring system. The O2 electrode within the water-jacketed cuvette may be used for measurements of O2 consumption (or production) by biological samples or enzymatic reactions. Calibration is a simple 2-point process, and all the requirements for calibration are supplied as part of the package.

Applications

Experiments Include:

Measurement of Aquatic Photosynthetic O2 Evolution
Effect of Inhibitors on Mitochondrial Electron Transport
Analysis of Light-dependent Chloroplast Electron Transport
Effects of Temperature on O2 Uptake by Aquatic Animals
Kinetics of O2-consuming Enzymes

Optional Components:

Syringes for addition of metabolites to the cuvette; (A141) 10µL, (A143) 50µL, A145) 100µL
S172 Thermistor (sample temperature)
A111 Variable halogen light source
S141 Light sensor

Experimental data showing the oxygen consumption rate of Glucose Oxidase using the OX1LP Dissolved Oxygen Package:

Other OX1LP Packages:

References:

Iris Bauer and Andreas Kappler. Rates and Extent of Reduction of Fe(III) Compounds and O₂ by Humic Substances. Environ. Sci. Technol. Vol 43, Number 13, p4902–4908 ( 2009).

Carrie M. Mosher, Matthew A. Hummel, Timothy S. Tracy and Allan E. Rettie. Functional Analysis of Phenylalanine Residues in the Active Site of Cytochrome P450 2C9. Biochemistry Vol 47, Number 45, p11725–11734 (2008).

Bryce A. Mendelsohn, Bethany L. Kassebaum and Jonathan D. Gitlin. The zebrafish embryo as a dynamic model of anoxia tolerance. Developmental Dynamics Vol 237, Issue 7, p1780–1788 (2008).

Bryce A. Mendelsohn and Jonathan D. Gitlin. Coordination of development and metabolism in the pre-midblastula transition zebrafish embryo. Developmental Dynamics Vol 237, Issue 7, p1789–1798 (2008).

Johnson EA, Rosenberg J, McCarty RE. Expression by Chlamydomonas reinhardtii of a chloroplast ATP synthease with polyhistidine-tagged beta subunits. Biochimica et Biophysica Acta 1767:374-380 (2007)

Johnson E A. Altered expression of the chloroplasts ATP synthase through site-directed mutagenesis in Chlamydamonas Reinhardtii. Photosynth Res vol 96:153-162 (2008)

Charles W. Locuson, Peter M. Gannett and Timothy S. Tracy. Heteroactivator effects on the coupling and spin state equilibrium of CYP2C9. Archives of Biochemistry and Biophysics Vol 449, Issues 1-2, p115-129 (2006).

OX1LP-6mL Dissolved O2 Package

admin — Wed, 14 Apr 2010 17:01:25 +0000

The OX1LP-6mL Dissolved Oxygen Package may be used to measure photosynthesis and/or respiration in the aqueous phase, using suspensions of plants, animals and organelles (chloroplasts and mitochondria). It may also be used to monitor chemical and biochemical reactions that either produce or consume O2 in the aqueous phase. The Dissolved Oxygen Package comes complete with instruments, software and interface.

The OX1LP-6mL Dissolved Oxygen Package includes:

G106 Dissolved Oxygen GLASS Cuvette Electrode (6 mL)
A231 Cuvette Electrode Amplifier
A255 Magnetic Stirrer
A135 Stir Bar (2) and O-Ring (2)
A131 KCl Filling Solution
A136 Membrane Material and Filter Paper
10 ft Excelon Tubing for the Water Jacket
C410 LabPro Interface
C901 Logger Pro Software
C404 Customized Setup Software
Instructor’s and Student’s Manuals

The OX1LP-6mL Dissolved Oxygen Package has features included in no other polarographic O2 monitoring system. The O2 electrode within the water-jacketed cuvette may be used for measurements of O2 consumption (or production) by biological samples or enzymatic reactions. Calibration is a simple 2-point process, and all the requirements for calibration are supplied as part of the package.

For many O2 electrodes, changing the membrane is a tedious and awkward process. Not so with Qubit Systems’ O2 electrode. The membrane is located under the cuvette, which screws onto the base plate, and requires only a few seconds to change. All materials for replacing the membrane (filter paper and membrane material) are included in the Dissolved Oxygen Package.

Applications

Experiments Include:

Measurement of Aquatic Photosynthetic O2 Evolution
Effect of Inhibitors on Mitochondrial Electron Transport
Analysis of Light-dependent Chloroplast Electron Transport
Effects of Temperature on O2 Uptake by Aquatic Animals
Kinetics of O2-consuming Enzymes

Optional Components:

Syringes for addition of metabolites to cuvette; (A141) 10µL, (A143) 50µL, A145) 100µL
S172 Thermistor (sample temperature)
A111 Variable halogen light source
S141 Light sensor

Experimental data showing the oxygen consumption rate of Glucose Oxidase using the OX1LP Dissolved Oxygen Package:

Other OX1LP Packages:

References:

Iris Bauer and Andreas Kappler. Rates and Extent of Reduction of Fe(III) Compounds and O₂ by Humic Substances. Environ. Sci. Technol. Vol 43, Number 13, p4902–4908 ( 2009).

Carrie M. Mosher, Matthew A. Hummel, Timothy S. Tracy and Allan E. Rettie. Functional Analysis of Phenylalanine Residues in the Active Site of Cytochrome P450 2C9. Biochemistry Vol 47, Number 45, p11725–11734 (2008).

Bryce A. Mendelsohn, Bethany L. Kassebaum and Jonathan D. Gitlin. The zebrafish embryo as a dynamic model of anoxia tolerance. Developmental Dynamics Vol 237, Issue 7, p1780–1788 (2008).

Bryce A. Mendelsohn and Jonathan D. Gitlin. Coordination of development and metabolism in the pre-midblastula transition zebrafish embryo. Developmental Dynamics Vol 237, Issue 7, p1789–1798 (2008).

Johnson EA, Rosenberg J, McCarty RE. Expression by Chlamydomonas reinhardtii of a chloroplast ATP synthease with polyhistidine-tagged beta subunits. Biochimica et Biophysica Acta 1767:374-380 (2007)

Johnson E A. Altered expression of the chloroplasts ATP synthase through site-directed mutagenesis in Chlamydamonas Reinhardtii. Photosynth Res vol 96:153-162 (2008)

Charles W. Locuson, Peter M. Gannett and Timothy S. Tracy. Heteroactivator effects on the coupling and spin state equilibrium of CYP2C9. Archives of Biochemistry and Biophysics Vol 449, Issues 1-2, p115-129 (2006).

OX1LP-4mL Dissolved O2 Package

admin — Wed, 14 Apr 2010 16:51:12 +0000

The OX1LP-4mL Dissolved Oxygen Package may be used to measure photosynthesis and/or respiration in the aqueous phase, using suspensions of plants, animals and organelles (chloroplasts and mitochondria). It may also be used to monitor chemical and biochemical reactions that either produce or consume O2 in the aqueous phase. The Dissolved Oxygen Package comes complete with instruments, software and interface.

The OX1LP-4mL Dissolved Oxygen Package includes:

G110 Dissolved Oxygen Cuvette Electrode (4 mL)
A231 Cuvette Electrode Amplifier
A255 Magnetic Stirrer
A135 Stir Bar (2) and O-Ring (2)
A131 KCl Filling Solution
A136 Membrane Material and Filter Paper
10 ft Excelon Tubing for the Water Jacket
C410 LabPro Interface
C901 Logger Pro Software
C404 Customized Setup Software
Instructor’s and Student’s Manuals

The OX1LP-4mL Dissolved Oxygen Package has features included in no other polarographic O2 monitoring system. The O2 electrode within the water-jacketed cuvette may be used for measurements of O2 consumption (or production) by biological samples or enzymatic reactions. Calibration is a simple 2-point process, and all the requirements for calibration are supplied as part of the package.

Applications

The Dissolved O2 Package may be used for any application that involves measurement of O2 in aqueous solutions. It can be used in the laboratory for measurement of O2 concentrations from samples taken from rivers and lakes, or to measure O2 consumption (or production) by living organisms. The Dissolved O2 Package is a perfect complement to Qubit Systems’ Respiration, Plant CO2 Analysis and Photosynthesis Packagess. While these packages are designed to measure the exchange of respiratory and photosynthetic gases in the gas phase, the O2 electrode allows similar measurements to be made on aquatic organisms, and also on isolated organelles, such as mitochondria and chloroplasts.

Experiments Include:

Measurement of Aquatic Photosynthetic O2 Evolution
Effect of Inhibitors on Mitochondrial Electron Transport
Analysis of Light-dependent Chloroplast Electron Transport
Effects of Temperature on O2 Uptake by Aquatic Animals
Kinetics of O2-consuming Enzymes

Optional Components:

Syringes for addition of metabolites to cuvette; (A141) 10µL, (A143) 50µL, (A145) 100µL
S172 Thermistor for measurements of sample temperature
A111 Variable halogen light source
S141 Light sensor

Experimental data showing the oxygen consumption rate of Glucose Oxidase using the OX1LP Dissolved Oxygen Package:

Other OX1LP Packages:

References:

Iris Bauer and Andreas Kappler. Rates and Extent of Reduction of Fe(III) Compounds and O₂ by Humic Substances. Environ. Sci. Technol. Vol 43, Number 13, p4902–4908 ( 2009).

Carrie M. Mosher, Matthew A. Hummel, Timothy S. Tracy and Allan E. Rettie. Functional Analysis of Phenylalanine Residues in the Active Site of Cytochrome P450 2C9. Biochemistry Vol 47, Number 45, p11725–11734 (2008).

Bryce A. Mendelsohn, Bethany L. Kassebaum and Jonathan D. Gitlin. The zebrafish embryo as a dynamic model of anoxia tolerance. Developmental Dynamics Vol 237, Issue 7, p1780–1788 (2008).

Bryce A. Mendelsohn and Jonathan D. Gitlin. Coordination of development and metabolism in the pre-midblastula transition zebrafish embryo. Developmental Dynamics Vol 237, Issue 7, p1789–1798 (2008).

Johnson EA, Rosenberg J, McCarty RE. Expression by Chlamydomonas reinhardtii of a chloroplast ATP synthease with polyhistidine-tagged beta subunits. Biochimica et Biophysica Acta 1767:374-380 (2007)

Johnson E A. Altered expression of the chloroplasts ATP synthase through site-directed mutagenesis in Chlamydamonas Reinhardtii. Photosynth Res vol 96:153-162 (2008)

Charles W. Locuson, Peter M. Gannett and Timothy S. Tracy. Heteroactivator effects on the coupling and spin state equilibrium of CYP2C9. Archives of Biochemistry and Biophysics Vol 449, Issues 1-2, p115-129 (2006).

OX1LP-1mL Dissolved O2 Package

admin — Wed, 14 Apr 2010 16:44:20 +0000

The OX1LP-1mL Dissolved Oxygen Package may be used to measure photosynthesis and/or respiration in the aqueous phase, using suspensions of plants, animals and organelles (chloroplasts and mitochondria). It may also be used to monitor chemical and biochemical reactions that either produce or consume O2 in the aqueous phase. The Dissolved Oyxgen Package comes complete with instruments, software and interface.

The OX1LP-1mL Dissolved Oxygen Package includes:

G108 Dissolved Oxygen Cuvette Electrode (1 mL)
A231 Cuvette Electrode Amplifier
A255 Magnetic Stirrer
A135 Stir Bar (2) and O-Ring (2)
A131 KCl Filling Solution
A136 Membrane Material and Filter Paper
10 ft Excelon Tubing for the Water Jacket
C410 LabPro Interface
C901 Logger Pro Software
C404 Customized Setup Software
Instructor’s and Student’s Manuals

The OX1LP-1mL Dissolved Oxygen Package has features included in no other polarographic O2 monitoring system. The O2 electrode within the water-jacketed cuvette may be used for measurements of O2 consumption (or production) by biological samples or enzymatic reactions. Calibration is a simple 2-point process, and all the requirements for calibration are supplied as part of the package.

Applications:

Experiments Include:

Measurement of Aquatic Photosynthetic O2 Evolution
Effect of Inhibitors on Mitochondrial Electron Transport
Analysis of Light-dependent Chloroplast Electron Transport
Effects of Temperature on O2 Uptake by Aquatic Animals
Kinetics of O2-consuming Enzymes

Optional Components:

Syringes for addition of metabolites to cuvette; (A141) 10µL, (A143) 50µL, (A145) 100µL
A111 Variable halogen light source
S141 Light sensor
S172 Thermistor is not available with the OX1LP-1mL package due to size constraints

Experimental data showing the oxygen consumption rate of Glucose Oxidase using the OX1LP Dissolved Oxygen Package:

Other OX1LP Packages:

References:

Sokolova IM, Sokolov EP, Ponnappa KM. Cadmium exposure affects mitochondrial bioenergetics and gene expression of key mitochondrial proteins in the eastern oyster Crassostrea virginica Gmelin (Bivalvia:Ostreidae). Aquatic Toxicology 73:241-255 (2005)
Lanniga G, Cherkasova AS, Sokolova IM. Temperature-dependent effects of cadmium on mitochondrial and whole-organism bioenergetics of oysters. Marine Environmental Research (Apr 2006)
Iris Bauer and Andreas Kappler. Rates and Extent of Reduction of Fe(III) Compounds and O₂ by Humic Substances. Environ. Sci. Technol. Vol 43, Number 13, p4902–4908 ( 2009).
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FL23 Algal CO2 Package (30mL)

admin — Tue, 13 Apr 2010 21:46:50 +0000

The FL23 Algal CO2 Analysis Package is used to measure CO2 exchange from algae in a plexiglass cuvette (30ml) designed by Qubit Systems. The CO2 gas released into head space of the cuvette is measured by the IR CO2 analyzer.

The FL23 Algal CO2 Exchange Package includes:

G103 DC Gas Pump
G122 Large Gas Bags
F250 Flow Meter
G214 Algal Chamber (30 mL)
S151 CO2 Analyzer (0 – 2000 ppm) (includes CO2 and H20 scrubbers)
C410 LabPro data acquisition interface
C901 LoggerPro data acquisition software

Gas enters the cuvette via the inlet port which is below the liquid level, causing a bubbling through the sample, and thus generates a mixing action. The gas outlet must be kept above the liquid line to prevent liquid from escaping the cuvette. Air coming out of the cuvette is sent through a drying column en route to S151 CO2 analyzer to measure CO2 concentration in the effluent gas stream.

Additional options:

The illumination of the algal culture can be provided by the A111 Halogen Light source or A113 LED light Source. Chlorophyll Fluorescence measurements can be obtained usign the Cuvette AquaPen (Z985) where the suspension of the algal culture is transferred into a cuvette for the measurements.

FL22 Algal CO2 Package (10mL)

admin — Tue, 13 Apr 2010 21:42:59 +0000

The FL22 Algal CO2 Analysis Package is used to measure CO2 exchange from algae in a plexiglass cuvette (10ml) designed by Qubit Systems. The CO2 gas released into head space of the cuvette is measured by the IR CO2 analyzer.

The FL22 Algal CO2 Exchange Package includes:

G103 DC Gas Pump
G122 Large Gas Bags
F250 Flow Meter
G213 Algal Chamber (10 mL)
S151 CO2 Analyzer (0 – 2000 ppm) (includes CO2 and H20 scrubbers)
C410 LabPro data acquisition interface
C901 LoggerPro data acquisition software

Gas enters the cuvette via the inlet port which is below the liquid level, causing a bubbling through the sample, and thus generates a mixing action. The gas outlet must be kept above the liquid line to prevent liquid from escaping the cuvette. Air coming out of the cuvette is sent through a drying column en route to the S151 CO2 analyzer to measure CO2 concentration in the effluent gas stream.

Additional options: