The OX1LP-50mL Dissolved Oxygen Package includes:

• G107 Dissolved Oxygen Cuvette Electrode (50 mL)
• A231 Cuvette Electrode Amplifier
• A255 Magnetic Stirrer
• A135 Stir Bar (2) and O-Ring (2)
• A131 KCl Filling Solution
• A136 Membrane Material and Filter Paper
• 10 ft Excelon Tubing for the Water Jacket
• C410 LabPro Interface
• C901 Logger Pro Software
• C404 Customized Setup Software
• Instructor’s and Student’s Manuals

The OX1LP-50mL Dissolved Oxygen Package has features included in no other polarographic O2 monitoring system. The O2 electrode within the water-jacketed cuvette may be used for measurements of O2 consumption (or production) by biological samples or enzymatic reactions. Calibration is a simple 2-point process, and all the requirements for calibration are supplied as part of the package.

For many O2 electrodes, changing the membrane is a tedious and awkward process. Not so with Qubit Systems’s O2 electrode. The membrane is located under the cuvette, which screws onto the base plate, and requires only a few seconds to change. All materials for replacing the membrane (filter paper and membrane material) are included in the Dissolved Oxygen Package.

Using C901 Logger Pro software, the O2 electrode may be calibrated in units of mg O2/L, % dissolved O2, ppm, or whatever units are appropriate for your study. The Dissolved Oxygen Package provides excellent accuracy and a resolution of 0.1% O2 (where air saturated water = 100%). Dissolved O2 values may be expressed in any desired units (mg/L; % dissolved O2; ppm etc) based on a simple calibration procedure. The water jacket allows calibrations and experiments to be carried out at the same temperature, or a simple temperature correction can be made in software for measurements made at different temperatures.

Applications

The Dissolved Oxygen Package may be used for any application that involves measurement of O2 in aqueous solutions. It can be used in the laboratory for measurement of O2 concentrations from samples taken from rivers and lakes, or to measure O2 consumption (or production) by living organisms. The Dissolved O2 Package is a perfect complement to Qubit Systems’ Respiration, Plant CO2 Analysis and Photosynthesis Packages. While these packages are designed to measure the exchange of respiratory and photosynthetic gases in the gas phase, the O2 electrode allows similar measurements to be made on aquatic organisms, and also on isolated organelles, such as mitochondria and chloroplasts.

Experiments Include:

• Measurement of Aquatic Photosynthetic O2 Evolution
• Effect of Inhibitors on Mitochondrial Electron Transport
• Analysis of Light-dependent Chloroplast Electron Transport
• Effects of Temperature on O2 Uptake by Aquatic Animals
• Kinetics of O2-consuming Enzymes

Optional Components:

• Syringes for addition of metabolites to cuvette; (A141) 10µL, (A143) 50µL, (A145) 100µL
• S172 Thermistor to measure cuvette sample temperature
• A111 Variable halogen light source
• S141 Light sensor

Experimental data showing the oxygen consumption rate of Glucose Oxidase using the OX1LP Dissolved Oxygen Package:

Other OX1LP Packages:

• OX1LP-1ml
• OX1LP-4ml
• OX1LP-6ml
• OX1LP-30ml

References:

• Iris Bauer and Andreas Kappler. Rates and Extent of Reduction of Fe(III) Compounds and O₂ by Humic Substances. Environ. Sci. Technol. Vol 43, Number 13, p4902–4908 ( 2009).

• Carrie M. Mosher, Matthew A. Hummel, Timothy S. Tracy and Allan E.  Rettie. Functional Analysis of Phenylalanine Residues in the Active Site of Cytochrome P450 2C9. Biochemistry Vol 47, Number 45, p11725–11734 (2008).

• Bryce A. Mendelsohn, Bethany L. Kassebaum and Jonathan D. Gitlin. The zebrafish embryo as a dynamic model of anoxia tolerance.  Developmental Dynamics Vol 237, Issue 7, p1780–1788 (2008).

• Bryce A. Mendelsohn and Jonathan D. Gitlin. Coordination of development and metabolism in the pre-midblastula transition zebrafish embryo.  Developmental Dynamics Vol 237, Issue 7, p1789–1798 (2008).

• Johnson EA, Rosenberg J, McCarty RE.  Expression by Chlamydomonas reinhardtii of a chloroplast ATP synthease with polyhistidine-tagged beta subunits.  Biochimica et Biophysica Acta 1767:374-380 (2007)

• Johnson E A. Altered expression of the chloroplasts ATP synthase through site-directed mutagenesis in Chlamydamonas Reinhardtii.  Photosynth Res vol 96:153-162 (2008)

• Charles W. Locuson, Peter M. Gannett and Timothy S. Tracy. Heteroactivator effects on the coupling and spin state equilibrium of CYP2C9. Archives of Biochemistry and Biophysics Vol 449, Issues 1-2, p115-129 (2006).

The OX1LP-30mL Dissolved Oxygen Package includes:

• G109 Dissolved Oxygen Cuvette Electrode (30 mL)
• A231 Cuvette Electrode Amplifier
• A255 Magnetic Stirrer
• A135 Stir Bar (2) and O-Ring (2)
• A131 KCl Filling Solution
• A136 Membrane Material and Filter Paper
• 10 ft Excelon Tubing for the Water Jacket
• C410 LabPro Interface
• C901 Logger Pro Software
• C404 Customized Setup Software
• Instructor’s and Student’s Manuals

The OX1LP-30mL Dissolved Oxygen Package has features included in no other polarographic O2 monitoring system. The O2 electrode within the water-jacketed cuvette may be used for measurements of O2 consumption (or production) by biological samples or enzymatic reactions. Calibration is a simple 2-point process, and all the requirements for calibration are supplied as part of the package.

For many O2 electrodes, changing the membrane is a tedious and awkward process. Not so with Qubit Systems’s O2 electrode. The membrane is located under the cuvette, which screws onto the base plate, and requires only a few seconds to change. All materials for replacing the membrane (filter paper and membrane material) are included in the Dissolved Oxygen Package.

Using C901 Logger Pro software, the O2 electrode may be calibrated in units of mg O2/L, % dissolved O2, ppm, or whatever units are appropriate for your study. The Dissolved Oxygen Package provides excellent accuracy and a resolution of 0.1% O2 (where air saturated water = 100%). Dissolved O2 values may be expressed in any desired units (mg/L; % dissolved O2; ppm etc) based on a simple calibration procedure. The water jacket allows calibrations and experiments to be carried out at the same temperature, or a simple temperature correction can be made in software for measurements made at different temperatures.

Applications

The Dissolved Oxygen Package may be used for any application that involves measurement of O2 in aqueous solutions. It can be used in the laboratory for measurement of O2 concentrations from samples taken from rivers and lakes, or to measure O2 consumption (or production) by living organisms. The Dissolved O2 Package is a perfect complement to Qubit Systems’ Respiration, Plant CO2 Analysis and Photosynthesis Packages. While these packages are designed to measure the exchange of respiratory and photosynthetic gases in the gas phase, the O2 electrode allows similar measurements to be made on aquatic organisms, and also on isolated organelles, such as mitochondria and chloroplasts.

Experiments Include:

• Measurement of Aquatic Photosynthetic O2 Evolution
• Effect of Inhibitors on Mitochondrial Electron Transport
• Analysis of Light-dependent Chloroplast Electron Transport
• Effects of Temperature on O2 Uptake by Aquatic Animals
• Kinetics of O2-consuming Enzymes

Optional Components:

• Syringes for addition of metabolites to the cuvette; (A141) 10µL, (A143) 50µL, A145) 100µL
• S172 Thermistor (sample temperature)
• A111 Variable halogen light source
• S141 Light sensor

Experimental data showing the oxygen consumption rate of Glucose Oxidase using the OX1LP Dissolved Oxygen Package:

Other OX1LP Packages:

• OX1LP-1ml
• OX1LP-4ml
• OX1LP-6ml
• OX1LP-50ml

References:

• Iris Bauer and Andreas Kappler. Rates and Extent of Reduction of Fe(III) Compounds and O₂ by Humic Substances. Environ. Sci. Technol. Vol 43, Number 13, p4902–4908 ( 2009).

• Carrie M. Mosher, Matthew A. Hummel, Timothy S. Tracy and Allan E.  Rettie. Functional Analysis of Phenylalanine Residues in the Active Site of Cytochrome P450 2C9. Biochemistry Vol 47, Number 45, p11725–11734 (2008).

• Bryce A. Mendelsohn, Bethany L. Kassebaum and Jonathan D. Gitlin. The zebrafish embryo as a dynamic model of anoxia tolerance.  Developmental Dynamics Vol 237, Issue 7, p1780–1788 (2008).

• Bryce A. Mendelsohn and Jonathan D. Gitlin. Coordination of development and metabolism in the pre-midblastula transition zebrafish embryo.  Developmental Dynamics Vol 237, Issue 7, p1789–1798 (2008).

• Johnson EA, Rosenberg J, McCarty RE.  Expression by Chlamydomonas reinhardtii of a chloroplast ATP synthease with polyhistidine-tagged beta subunits.  Biochimica et Biophysica Acta 1767:374-380 (2007)

• Johnson E A. Altered expression of the chloroplasts ATP synthase through site-directed mutagenesis in Chlamydamonas Reinhardtii.  Photosynth Res vol 96:153-162 (2008)

• Charles W. Locuson, Peter M. Gannett and Timothy S. Tracy. Heteroactivator effects on the coupling and spin state equilibrium of CYP2C9. Archives of Biochemistry and Biophysics Vol 449, Issues 1-2, p115-129 (2006).

The OX1LP-6mL Dissolved Oxygen Package includes:

• G106 Dissolved Oxygen GLASS Cuvette Electrode (6 mL)
• A231 Cuvette Electrode Amplifier
• A255 Magnetic Stirrer
• A135 Stir Bar (2) and O-Ring (2)
• A131 KCl Filling Solution
• A136 Membrane Material and Filter Paper
• 10 ft Excelon Tubing for the Water Jacket
• C410 LabPro Interface
• C901 Logger Pro Software
• C404 Customized Setup Software
• Instructor’s and Student’s Manuals

The OX1LP-6mL Dissolved Oxygen Package has features included in no other polarographic O2 monitoring system. The O2 electrode within the water-jacketed cuvette may be used for measurements of O2 consumption (or production) by biological samples or enzymatic reactions. Calibration is a simple 2-point process, and all the requirements for calibration are supplied as part of the package.

For many O2 electrodes, changing the membrane is a tedious and awkward process. Not so with Qubit Systems’  O2 electrode. The membrane is located under the cuvette, which screws onto the base plate, and requires only a few seconds to change. All materials for replacing the membrane (filter paper and membrane material) are included in the Dissolved Oxygen Package.

Using C901 Logger Pro software, the O2 electrode may be calibrated in units of mg O2/L, % dissolved O2, ppm, or whatever units are appropriate for your study. The Dissolved Oxygen Package provides excellent accuracy and a resolution of 0.1% O2 (where air saturated water = 100%). Dissolved O2 values may be expressed in any desired units (mg/L; % dissolved O2; ppm etc) based on a simple calibration procedure. The water jacket allows calibrations and experiments to be carried out at the same temperature, or a simple temperature correction can be made in software for measurements made at different temperatures.

Applications

The Dissolved Oxygen Package may be used for any application that involves measurement of O2 in aqueous solutions. It can be used in the laboratory for measurement of O2 concentrations from samples taken from rivers and lakes, or to measure O2 consumption (or production) by living organisms. The Dissolved O2 Package is a perfect complement to Qubit Systems’ Respiration, Plant CO2 Analysis and Photosynthesis Packages. While these packages are designed to measure the exchange of respiratory and photosynthetic gases in the gas phase, the O2 electrode allows similar measurements to be made on aquatic organisms, and also on isolated organelles, such as mitochondria and chloroplasts.

Experiments Include:

• Measurement of Aquatic Photosynthetic O2 Evolution
• Effect of Inhibitors on Mitochondrial Electron Transport
• Analysis of Light-dependent Chloroplast Electron Transport
• Effects of Temperature on O2 Uptake by Aquatic Animals
• Kinetics of O2-consuming Enzymes

Optional Components:

• Syringes for addition of metabolites to cuvette; (A141) 10µL, (A143) 50µL, A145) 100µL
• S172 Thermistor (sample temperature)
• A111 Variable halogen light source
• S141 Light sensor

Experimental data showing the oxygen consumption rate of Glucose Oxidase using the OX1LP Dissolved Oxygen Package:

Other OX1LP Packages:

• OX1LP-1ml
• OX1LP-4ml
• OX1LP-30ml
• OX1LP-50ml

References:

• Iris Bauer and Andreas Kappler. Rates and Extent of Reduction of Fe(III) Compounds and O₂ by Humic Substances. Environ. Sci. Technol. Vol 43, Number 13, p4902–4908 ( 2009).

• Carrie M. Mosher, Matthew A. Hummel, Timothy S. Tracy and Allan E.  Rettie. Functional Analysis of Phenylalanine Residues in the Active Site of Cytochrome P450 2C9. Biochemistry Vol 47, Number 45, p11725–11734 (2008).

• Bryce A. Mendelsohn, Bethany L. Kassebaum and Jonathan D. Gitlin. The zebrafish embryo as a dynamic model of anoxia tolerance.  Developmental Dynamics Vol 237, Issue 7, p1780–1788 (2008).

• Bryce A. Mendelsohn and Jonathan D. Gitlin. Coordination of development and metabolism in the pre-midblastula transition zebrafish embryo.  Developmental Dynamics Vol 237, Issue 7, p1789–1798 (2008).

• Johnson EA, Rosenberg J, McCarty RE.  Expression by Chlamydomonas reinhardtii of a chloroplast ATP synthease with polyhistidine-tagged beta subunits.  Biochimica et Biophysica Acta 1767:374-380 (2007)

• Johnson E A. Altered expression of the chloroplasts ATP synthase through site-directed mutagenesis in Chlamydamonas Reinhardtii.  Photosynth Res vol 96:153-162 (2008)

• Charles W. Locuson, Peter M. Gannett and Timothy S. Tracy. Heteroactivator effects on the coupling and spin state equilibrium of CYP2C9. Archives of Biochemistry and Biophysics Vol 449, Issues 1-2, p115-129 (2006).

The OX1LP-4mL Dissolved Oxygen Package includes:

• G110 Dissolved Oxygen Cuvette Electrode (4 mL)
• A231 Cuvette Electrode Amplifier
• A255 Magnetic Stirrer
• A135 Stir Bar (2) and O-Ring (2)
• A131 KCl Filling Solution
• A136 Membrane Material and Filter Paper
• 10 ft Excelon Tubing for the Water Jacket
• C410 LabPro Interface
• C901 Logger Pro Software
• C404 Customized Setup Software
• Instructor’s and Student’s Manuals

The OX1LP-4mL Dissolved Oxygen Package has features included in no other polarographic O2 monitoring system. The O2 electrode within the water-jacketed cuvette may be used for measurements of O2 consumption (or production) by biological samples or enzymatic reactions. Calibration is a simple 2-point process, and all the requirements for calibration are supplied as part of the package.

For many O2 electrodes, changing the membrane is a tedious and awkward process. Not so with Qubit Systems’ O2 electrode. The membrane is located under the cuvette, which screws onto the base plate, and requires only a few seconds to change. All materials for replacing the membrane (filter paper and membrane material) are included in the Dissolved Oxygen Package.

Using C901 Logger Pro software, the O2 electrode may be calibrated in units of mg O2/L, % dissolved O2, ppm, or whatever units are appropriate for your study. The Dissolved Oxygen Package provides excellent accuracy and a resolution of 0.1% O2 (where air saturated water = 100%). Dissolved O2 values may be expressed in any desired units (mg/L; % dissolved O2; ppm etc) based on a simple calibration procedure. The water jacket allows calibrations and experiments to be carried out at the same temperature, or a simple temperature correction can be made in software for measurements made at different temperatures.

Applications

The Dissolved O2 Package may be used for any application that involves measurement of O2 in aqueous solutions. It can be used in the laboratory for measurement of O2 concentrations from samples taken from rivers and lakes, or to measure O2 consumption (or production) by living organisms. The Dissolved O2 Package is a perfect complement to Qubit Systems’ Respiration, Plant CO2 Analysis and Photosynthesis Packagess. While these packages are designed to measure the exchange of respiratory and photosynthetic gases in the gas phase, the O2 electrode allows similar measurements to be made on aquatic organisms, and also on isolated organelles, such as mitochondria and chloroplasts.

Experiments Include:

• Measurement of Aquatic Photosynthetic O2 Evolution
• Effect of Inhibitors on Mitochondrial Electron Transport
• Analysis of Light-dependent Chloroplast Electron Transport
• Effects of Temperature on O2 Uptake by Aquatic Animals
• Kinetics of O2-consuming Enzymes

Optional Components:

• Syringes for addition of metabolites to cuvette; (A141) 10µL, (A143) 50µL, (A145) 100µL
• S172 Thermistor for measurements of sample temperature
• A111 Variable halogen light source
• S141 Light sensor

Experimental data showing the oxygen consumption rate of Glucose Oxidase using the OX1LP Dissolved Oxygen Package:

Other OX1LP Packages:

• OX1LP-1ml
• OX1LP-30ml
• OX1LP-6ml
• OX1LP-50ml

References:

• Iris Bauer and Andreas Kappler. Rates and Extent of Reduction of Fe(III) Compounds and O₂ by Humic Substances. Environ. Sci. Technol. Vol 43, Number 13, p4902–4908 ( 2009).

• Carrie M. Mosher, Matthew A. Hummel, Timothy S. Tracy and Allan E.  Rettie. Functional Analysis of Phenylalanine Residues in the Active Site of Cytochrome P450 2C9. Biochemistry Vol 47, Number 45, p11725–11734 (2008).

• Bryce A. Mendelsohn, Bethany L. Kassebaum and Jonathan D. Gitlin. The zebrafish embryo as a dynamic model of anoxia tolerance.  Developmental Dynamics Vol 237, Issue 7, p1780–1788 (2008).

• Bryce A. Mendelsohn and Jonathan D. Gitlin. Coordination of development and metabolism in the pre-midblastula transition zebrafish embryo.  Developmental Dynamics Vol 237, Issue 7, p1789–1798 (2008).

• Johnson EA, Rosenberg J, McCarty RE.  Expression by Chlamydomonas reinhardtii of a chloroplast ATP synthease with polyhistidine-tagged beta subunits.  Biochimica et Biophysica Acta 1767:374-380 (2007)

• Johnson E A. Altered expression of the chloroplasts ATP synthase through site-directed mutagenesis in Chlamydamonas Reinhardtii.  Photosynth Res vol 96:153-162 (2008)

• Charles W. Locuson, Peter M. Gannett and Timothy S. Tracy. Heteroactivator effects on the coupling and spin state equilibrium of CYP2C9. Archives of Biochemistry and Biophysics Vol 449, Issues 1-2, p115-129 (2006).

The OX1LP-1mL Dissolved Oxygen Package includes:

• G108 Dissolved Oxygen Cuvette Electrode (1 mL)
• A231 Cuvette Electrode Amplifier
• A255 Magnetic Stirrer
• A135 Stir Bar (2) and O-Ring (2)
• A131 KCl Filling Solution
• A136 Membrane Material and Filter Paper
• 10 ft Excelon Tubing for the Water Jacket
• C410 LabPro Interface
• C901 Logger Pro Software
• C404 Customized Setup Software
• Instructor’s and Student’s Manuals

The OX1LP-1mL Dissolved Oxygen Package has features included in no other polarographic O2 monitoring system. The O2 electrode within the water-jacketed cuvette may be used for measurements of O2 consumption (or production) by biological samples or enzymatic reactions. Calibration is a simple 2-point process, and all the requirements for calibration are supplied as part of the package.

For many O2 electrodes, changing the membrane is a tedious and awkward process. Not so with Qubit Systems’ O2 electrode. The membrane is located under the cuvette, which screws onto the base plate, and requires only a few seconds to change. All materials for replacing the membrane (filter paper and membrane material) are included in the Dissolved Oxygen Package.

Using C901 Logger Pro software, the O2 electrode may be calibrated in units of mg O2/L, % dissolved O2, ppm, or whatever units are appropriate for your study. The Dissolved Oxygen Package provides excellent accuracy and a resolution of 0.1% O2 (where air saturated water = 100%). Dissolved O2 values may be expressed in any desired units (mg/L; % dissolved O2; ppm etc) based on a simple calibration procedure. The water jacket allows calibrations and experiments to be carried out at the same temperature, or a simple temperature correction can be made in software for measurements made at different temperatures.

Applications:

The Dissolved Oxygen Package may be used for any application that involves measurement of O2 in aqueous solutions. It can be used in the laboratory for measurement of O2 concentrations from samples taken from rivers and lakes, or to measure O2 consumption (or production) by living organisms. The Dissolved O2 Package is a perfect complement to Qubit Systems’ Respiration, Plant CO2 Analysis and Photosynthesis Packages. While these packages are designed to measure the exchange of respiratory and photosynthetic gases in the gas phase, the O2 electrode allows similar measurements to be made on aquatic organisms, and also on isolated organelles, such as mitochondria and chloroplasts.

Experiments Include:

• Measurement of Aquatic Photosynthetic O2 Evolution
• Effect of Inhibitors on Mitochondrial Electron Transport
• Analysis of Light-dependent Chloroplast Electron Transport
• Effects of Temperature on O2 Uptake by Aquatic Animals
• Kinetics of O2-consuming Enzymes

Optional Components:

• Syringes for addition of metabolites to cuvette; (A141) 10µL, (A143) 50µL, (A145) 100µL
• A111 Variable halogen light source
• S141 Light sensor
• S172 Thermistor is not available with the OX1LP-1mL package due to size constraints

Experimental data showing the oxygen consumption rate of Glucose Oxidase using the OX1LP Dissolved Oxygen Package:

Other OX1LP Packages:

• OX1LP-4ml
• OX1LP-6ml
• OX1LP-30ml
• OX1LP-50ml

References:

• Sokolova IM, Sokolov EP, Ponnappa KM. Cadmium exposure affects mitochondrial bioenergetics and gene expression of key mitochondrial proteins in the eastern oyster Crassostrea virginica Gmelin (Bivalvia:Ostreidae).  Aquatic Toxicology 73:241-255 (2005)
• Lanniga G, Cherkasova AS, Sokolova IM. Temperature-dependent effects of cadmium on mitochondrial and whole-organism bioenergetics of oysters. Marine Environmental Research (Apr 2006)
• Iris Bauer and Andreas Kappler. Rates and Extent of Reduction of Fe(III) Compounds and O₂ by Humic Substances. Environ. Sci. Technol. Vol 43, Number 13, p4902–4908 ( 2009).
• Carrie M. Mosher, Matthew A. Hummel, Timothy S. Tracy and Allan E.  Rettie. Functional Analysis of Phenylalanine Residues in the Active Site of Cytochrome P450 2C9. Biochemistry Vol 47, Number 45, p11725–11734 (2008).
• Bryce A. Mendelsohn, Bethany L. Kassebaum and Jonathan D. Gitlin. The zebrafish embryo as a dynamic model of anoxia tolerance.  Developmental Dynamics Vol 237, Issue 7, p1780–1788 (2008).
• Bryce A. Mendelsohn and Jonathan D. Gitlin. Coordination of development and metabolism in the pre-midblastula transition zebrafish embryo.  Developmental Dynamics Vol 237, Issue 7, p1789–1798 (2008).
• Johnson EA, Rosenberg J, McCarty RE.  Expression by Chlamydomonas reinhardtii of a chloroplast ATP synthease with polyhistidine-tagged beta subunits.  Biochimica et Biophysica Acta 1767:374-380 (2007)
• Johnson E A. Altered expression of the chloroplasts ATP synthase through site-directed mutagenesis in Chlamydamonas Reinhardtii.  Photosynth Res vol 96:153-162 (2008)
• Charles W. Locuson, Peter M. Gannett and Timothy S. Tracy. Heteroactivator effects on the coupling and spin state equilibrium of CYP2C9. Archives of Biochemistry and Biophysics Vol 449, Issues 1-2, p115-129 (2006).

The AquaResp – Aquatic Respirometry Package measures oxygen production,  or consumption in aquatic plants and animals. The package is a single channel, open flow, intermittent respirometry system capable of measuring metabolic O2 exchange in small fish, invertebrates and even plants and algae!

The Aquatic Respirometry Package comes complete with quality sensors as well as PC software and USB instrument for data acquisition and pump control. The software provides real-time graphs and logs calibrated data into Excel files.

The Automated Respirometry Package includes:

• Respiration chamber (standard: ID=62mm x L=210mm)
• Flush pump (4.5 LPM) and recirculation pump (4.5 LPM)
• 15-bit USB instrument for data acquisition and pump control (shown at right)
• D901 Galvanic Oxygen Probe including maintenance kit and storage vessel
• Pt100 temperature probe
• PVC tubing for pumps and chamber
• Stainless steel wing nuts and washers for chamber)
• 24VAC power adapter (for 120V or 230V power sources)
• User manual

The AQUARESP System is based around a series of pumps, tubing and a respirometry chamber, specifically designed for the organism you wish to measure. There are 2 pumps, one controlling a flush period and the other a recirculation period. When the flush pump is on, the system is purged with water from an external reservoir, recharging the system with fresh water that has been equilibrating with atmospheric O2. When the flush pump turns off, the recirculation pump continuously recirculates water in a closed loop through the system.

Although the Aquatic Respirometry Package was not designed for swim tunnel experiments, you can adjust the flow rate from the recirculation pump to force fish to experience different flow speeds. The standard pump supplied with the package provides an unrestricted flow of 4.5 LPM, but provides a maximum of 3 LPM when connected to the system. Lower flow rates can be selected by adjusting an in-line valve in the pump circuit.

APPLICATIONS

• Illustrate effects of temperature, hypoxia, feeding, stress, body size etc. on cellular respiration of aquatic organisms.
• Show instant changes in primary production as light is turned on and off.
• Measure dissolved oxygen to examine effects of water pollution, e.g. thermal, biodegradable, and chemical.

FEATURES

• High quality (galvanic cell) oxygen probe for accurate and stable readings with a minimum of maintenance
• USB instrument controls the activity of experimental pumps for automated long-term measurements (hours-days)
• A replaceable Perpex tube allows you to change chamber volume and size to fit available animals or plants
• User manual with experimental procedures, theoretical background and exercises

Figure 1. Experimental run screen from AQUARESP.