The DS-TEMP Shuttle Box System for Temperature is an automated system for determining the preferred water temperature of aquatic invertebrates or fish.  It includes everything needed for video behavior analysis, data acquisition and digital control of devices.

Inside an experimental tank the animal can freely “shuttle” between two sub compartments with opposite acting controls, to regulate water temperature to preferred values through behavior.

The tank design allows for independent control of water quality in the two sub compartments, and is made special to match the type and size of your specimens.

Features:

• Turn-key system including everything needed
• ShuttleSoft software for Windows
• Research-grade video camera equipment
• Real-time video tracking and behavioral analysis
• USB data acquisition and digital relay control of pumps/valves
• Rugged temperature instruments and sensors
• Change settings and controls on-the-fly

Consider upgrading this system to also measure preferred water salinity or oxygen saturation (ask us for a quote).

Shuttle Box Setup

Included:

• Shuttle Box (customer-specified dimensions)
• ShuttleSoft software (one license for a limited number of PCs only)
• Ueye 1640-C video camera (1280×1024), lens and USB cable
• DAQ-S instrument for 16-bit data acquisition and digital relay control
• Two water temperature systems (excl. chiller/heater)
• Two accessory sets for temperature control
• Two inline probe chambers
• Two buffer tanks
• Pump, tubing and fittings
• User manual

Note: we offer special packages for (exposure) tests keeping water quality at set point levels during the experiment independent of fish behavior, and for multiple parameters, e.g. temperature and oxygen etc.

DS-Salinity Shuttle Box System is an automated system for determining the preferred water salinity of aquatic invertebrates or fish.  It includes everything needed for video behavior analysis, data acquisition and digital control of devices.

Inside an experimental tank the animal can freely “shuttle” between two sub compartments with opposite acting controls, to regulate water salinity to preferred values through behavior.

The tank design allows for independent control of water quality in the two sub compartments, and is made special to match the type and size of your specimens.

• Shuttle Box (customer-specified dimensions)
• ShuttleSoft software (one license for a limited number of PCs only)
• Ueye 1640-C video camera (1280×1024), lens and USB cable
• DAQ-S instrument for 16-bit data acquisition and digital relay control
• Two WTW 3310 conductivity instruments incl. TetraCon 325 probes
• Four pumps with tubing and fittings
• Two inline probe chambers
• Two buffer tanks
• Pumps, tubing and fittings
• User manual

NB: we offer special packages for (exposure) tests keeping water quality at set point levels during the experiment independent of fish behavior, and for multiple parameters, e.g. temperature and oxygen etc.

Features:

• Turn-key system including everything needed
• ShuttleSoft software for Windows
• Research-grade video camera equipment
• Real-time video tracking and behavioral analysis
• USB data acqusition and digital relay control of pumps/valves
• Rugged conductivity instruments and sensors form German WTW
• Change settings and controls on-the-fly

Consider upgrading this system to also measure preferred water temperature or oxygen (ask us for a quote).

Shuttle Box Setup

DS-OXYGEN Shuttle Box System is an automated system for determining the preferred oxygen saturation of aquatic invertebrates or fish.  It includes everything needed for video behavior analysis, data acquisition and digital control of devices.

Inside an experimental tank the animal can freely “shuttle” between two sub compartments with opposite acting controls, to regulate water oxygen saturation to preferred values through behavior.

The tank design allows for independent control of water quality in the two sub compartments, and is made special to match the type and size of your specimens.

Included:

• Shuttle Box (customer-specified dimensions)
• ShuttleSoft software (one license for a limited number of PCs only)
• DAQ-S instrument for 16-bit data acquisition and digital relay control
• Ueye 1640-C video camera (1280×1024), lens and USB cable
• Two D202 oxygen analyzer & regulator systems
• Two accessory sets for gas control
• Two inline oxygen probe chambers
• Two gas reactors
• Pumps, tubing, fittings
• User manual

NB: we offer special packages for (exposure) tests keeping water quality at set point levels during the experiment independent of fish behavior, and for multiple parameters, e.g. temperature and oxygen etc.

Features:

• Turn-key system including everything needed
• ShuttleSoft software for Windows
• Research-grade video camera equipment
• Real-time video tracking and behavioral analysis
• USB data acquisition and digital relay control of pumps/valves
• Rugged oxygen sensors and measuring instruments
• Change settings and controls on-the-fly

Consider upgrading this system to also measure preferred water temperature or salinity (ask us for a quote).

Shuttle Box Setup

This automated feedback system will determine pCO2 indirectly as water pH, and regulate pCO2 or pH to a level set by the user. The user-friendly windows software makes it possible to monitor calibrated values for pH and pCO2 in a real-time semi log plot (log pCO2 vs. pH), and control either to set point levels automatically.

With optional accessories this system will also regulate water temperature or pH/pCO2 in up to four different tanks or aquaria.

Applications range from studies of ocean acidification, water chemistry, effects of hypercapnia in aquatic organisms, and normocapnic control in recirculated or closed tanks and aquaria.

Included:

• WTW pH 3310 SET incl. SenTix 41-3 probe, buffers, suitcase and USB cable
• Digital relay instrument with USB interface (DAQ-S)
• Suitcase
• Solenoid valve
• Push-in fittings
• PU air tubing
• Air stone
• User manual

TYPICAL EXPERIMENTAL SETUP

Features:

• pCO2 is monitored indirectly as water pH, and regulated by injection of 100% CO2 gas or air
• A pH 3310 instrument and probe from German WTW is connected to your pc via USB
• User-friendly software for Windows, allows you to calibrate, graph, record and regulate water pH or pCO2 values to pre set values, e.g. for normocapnic or hypercapnic control
• USB instrument with build-in digital relays, complete with a solenoid valve and fittings, will control injection of CO2 gas or other gasses
• The relationship between pCO2 and pH is obtained by measuring pH in water equilibrated with two different pCO2 partial pressures, e.g. use a gas mixing pump (Woesthoff) or special lab gases

WTW pH 3310:                    pCO2 calibration:

Optional:

• SenTix H pH electrode + cable for measurements in sea water.
• D203 for water temperature regulation.
• One extra D202 and one extra WTW pH 3310 SET for independent pH/pCO2 regulation in one more tank/aquarium.

The following references used the same technique to control pCO2.

D.J. McKenzie, A.Z. Dalla Valle, M. Piccolella, E.W. Taylor and J.F. Steffensen. 2003. Tolerance of chronic hypercapnia by the European eel (Anguilla anguilla). J. exp. Biol. 206: 1717-1726.

D.J. McKenzie, E.W. Taylor, A.Z. Dalla Valle and J.F. Steffensen. 2002. Tolerance of acute hypercapnic acidosis by the European eel (Anguilla anguilla). J. Comp. Physiol. B 172: 347-354.

Cruz-Neto, A. and Steffensen, J. F. 1997. The effects of acute hypoxia and hypercapnia oxygen consumption of freshwater European eel, Anguilla anguilla L. J. Fish Biol. 50; 759-769.

This single-channel temperature system includes everything needed to monitor and control water temperature. The instrument’s analog output allow for temperature data acquisition.

A large display, two digital relays for valve control, menu-driven settings and an analog output for data acquisition, makes this system user friendly and well suited for a range of lab applications.

Example – Cold water control. The temperature set point value is compared with measured values. If the temperature is higher than the set point value the digital relay activates the pump which flushes cold water through a stainless steel coil inside the ambient water tank. When the temperature reaches the set-point, the pump is turned off.

Controller Specifications

System includes:

• Controller Instrument
• Pt100 Temperature probe (5 m)
• Stainless steel heat-exchanger coil
• Submersible pump, 10 L/min
• Soft PVC tubing (5m) and fittings
• Analog output cable for data acquisition (0-5 V)
• Adapter cable for pump
• Power cable
• User manual
• Carrying case

System excludes:

• Cooling bath/heater

The D202 Oxygen Regulation System is a complete system that will monitor and regulate oxygen saturation in sea or fresh water, e.g. for normoxic, hyperoxic or hypoxic control in fish tanks, aquariums etc.

A large display, two digital relays for valve control, menu-driven settings and an analog output for data acquisition, makes this system user friendly and well suited for a range of lab applications.

Example – Hypoxic control. The oxygen set point value is compared with measured values. If the oxygen saturation is higher than the set point value the digital relay activates the solenoid valve to open for injection of nitrogen gas (gas bottle not included).

System Includes:

• D201 controller instrument
• D901 Miniature Galvanic DO2 probe and maintenance kit
• Preamplifier with galvanic isolation
• Solenoid valve for O2 and N2 (24, 110 or 230V)
• Air tubing (6/4 mm)
• 1/8″ fittings
• Ceramic diffuser
• Analog output (0 – 5V)

System Excludes:

• Pressure bottle and regulator for N2 or O2

Suggested set up of D202:

Controller (D201) Specifications

IMPORTANT: DO NOT connect relays to >500W equipment (max 2 A, 250 V).

Mini-DO probe(D901) specifications:

D112 is a research-grade flow measuring equipment from Höntzsch, includes a Vane Wheel Flow Sensor and a Handheld HFA Flow Meter. This instrument is ideal for calibrating the motor inside a swim tunnel, or any other flowing water source.

Included:

• Digital flow meter
• Stainless vane wheel flow probe
• User manual

Vane Wheel Flow Sensor

• Material: Stainless steel
• Diameter: 30 mm
• Flow range: 0.01 – 3 m/s in depths of 20 – 150 mm
• Includes a probe extension for depths of 150 – 500 mm

Handheld HFA Flow Meter

• Hand-held digital flow instrument
• High-contrast LCD (liquid crystal display)
• RS-232 interface for direct transfer of stored values to a PC
• Instantaneous flow values every second
• Display units: m/s, m³/h, L/min (optional °C)
• Battery-operated by 9 V compound battery or alternatively by main supply circuit
• Analog output
• Data and measured value storage for 500 data records