• a custom-sized glass chamber of variable volume (500 – 5000 µl), inner diameter is 11.2 mm
• a 3-port glass stopper made for intermittent flow respirometry using fiber-optic needle-type sensors (O2 or pH) or other micro sensors.

Included:

• One custom-sized glass chamber
• One 3-port glass stopper
• One glass spacer
• One glass coated magnetic stir bar
• Stainless steel net for separation of magnet and fragile samples, 10 cm²

Features:

• Volumes in the range of 0.5 – 5 mL are available to fit different respiration or photosynthetic rates
• Designed for intermittent flow respirometry for long-term experiments with high temporal resolution and sensitivity
• No materials acting as oxygen sources or sinks
• Chamber and stopper made in borosilicate glass to give a tight seal and prevent diffusion of oxygen between the chamber and surroundings
• Designed for use with oxygen micro sensors featuring no O2 consumption, low response time, no stir effect, no cross-sensitivity, no electrical interference, sterilizable (H2O2, EtOH) etc.

NB: every chamber is handmade from borosilicate glass and therefore, their exact volumes have to be determined individually!

Measuring Principle:

One port is for the micro sensor and is made as an open capillary tube (0.75 ×14 mm) so tiny that it effectively prevents diffusion of air if filled with water or oil. The other two ports are for flushing the chamber intermittently with a peristaltic pump. Both of these are fitted with pieces of steel needle for tygon tubes.

Fragile samples are separated from the glass-coated magnetic stir bar, by placing a piece of stainless steel net (mesh size 250 µm) on top of a glass spacer O-ring.

Applications:

• Oxygen consumption of individual eggs, embryos and larvae (invertebrates, fish)
• Net oxygen evolution and respiration rates of phytoplankton cultures of low density
• Other applications with very low oxygen fluxes

• Use the respirometers with your own oxygen and data acquisition equipment or with our systems for automatic respirometry.
• Pumps are required for flushing and recirculating water in the chamber. We suggest pumps with a flow rate per minute five times the chamber volume (e.g. if chamber volume is 1 L, we recommend a 5 L/min pump).
• Tubing is required to connect the pumps with the respirometer.
• Replacement tubes are available, please contact us for details.

**35 and 45mm chambers made from glass

Use the respirometers with your own oxygen and data acquisition equipment or with our systems for automatic respirometry.

• Free choice of chamber length, and 8 different inner diameters
• Chambers made for use in full strength sea water
• Easy tool-free handling and cleaning
• Smaller chambers made of glass to avoid O2 storage/release
• Built-in baffles for even distribution of flow

• For flushing and recirculating the water in the chamber pumps are needed. We suggest pumps with a flow rate per minute five times the chamber volume (e.g. if chamber volume is 1 L, we recommend a 5 L/min pump).
• To connect the pumps with the respirometer tubing is needed.
• Place the oxygen probe in one of our inline flow-through chambers (D025, D026, D027).
• Replacement glass tubes are available, please contact us for details.

• Use the respirometers with your own oxygen and data acquisition equipment or with our systems for automatic respirometry.
• Pumps are required for flushing and recirculating water in the chamber. We suggest pumps with a flow rate per minute five times the chamber volume (e.g. if chamber volume is 1 L, we recommend a 5 L/min pump).
• Tubing is required to connect the pumps with the respirometer.
• Replacement tubes are available, please contact us for details.

**35 and 45mm chambers made from glass

Use the respirometers with your own oxygen and data acquisition equipment or with our systems for automatic respirometry.

• Free choice of chamber length, and 8 different inner diameters
• Chambers made for use in full strength sea water
• Easy tool-free handling and cleaning
• Smaller chambers made of glass to avoid O2 storage/release
• Built-in baffles for even distribution of flow

• For flushing and recirculating the water in the chamber pumps are needed. We suggest pumps with a flow rate per minute five times the chamber volume (e.g. if chamber volume is 1 L, we recommend a 5 L/min pump).
• To connect the pumps with the respirometer tubing is needed.
• Place the oxygen probe in one of our inline flow-through chambers (D025, D026, D027).
• Replacement glass tubes are available, please contact us for details.

• Use the respirometers with your own oxygen and data acquisition equipment or with our systems for automatic respirometry.
• Pumps are required for flushing and recirculating water in the chamber. We suggest pumps with a flow rate per minute five times the chamber volume (e.g. if chamber volume is 1 L, we recommend a 5 L/min pump).
• Tubing is required to connect the pumps with the respirometer.
• Replacement tubes are available, please contact us for details.

**35 and 45mm chambers made from glass

Use the respirometers with your own oxygen and data acquisition equipment or with our systems for automatic respirometry.

• Free choice of chamber length, and 8 different inner diameters
• Chambers made for use in full strength sea water
• Easy tool-free handling and cleaning
• Smaller chambers made of glass to avoid O2 storage/release
• Built-in baffles for even distribution of flow

• For flushing and recirculating the water in the chamber pumps are needed. We suggest pumps with a flow rate per minute five times the chamber volume (e.g. if chamber volume is 1 L, we recommend a 5 L/min pump).
• To connect the pumps with the respirometer tubing is needed.
• Place the oxygen probe in one of our inline flow-through chambers (D025, D026, D027).
• Replacement glass tubes are available, please contact us for details.

• Use the respirometers with your own oxygen and data acquisition equipment or with our systems for automatic respirometry.
• Pumps are required for flushing and recirculating water in the chamber. We suggest pumps with a flow rate per minute five times the chamber volume (e.g. if chamber volume is 1 L, we recommend a 5 L/min pump).
• Tubing is required to connect the pumps with the respirometer.
• Replacement tubes are available, please contact us for details.

**35 and 45mm chambers made from glass

Use the respirometers with your own oxygen and data acquisition equipment or with our systems for automatic respirometry.

• Free choice of chamber length, and 8 different inner diameters
• Chambers made for use in full strength sea water
• Easy tool-free handling and cleaning
• Smaller chambers made of glass to avoid O2 storage/release
• Built-in baffles for even distribution of flow

• For flushing and recirculating the water in the chamber pumps are needed. We suggest pumps with a flow rate per minute five times the chamber volume (e.g. if chamber volume is 1 L, we recommend a 5 L/min pump).
• To connect the pumps with the respirometer tubing is needed.
• Place the oxygen probe in one of our inline flow-through chambers (D025, D026, D027).
• Replacement glass tubes are available, please contact us for details.

• Use the respirometers with your own oxygen and data acquisition equipment or with our systems for automatic respirometry.
• Pumps are required for flushing and recirculating water in the chamber. We suggest pumps with a flow rate per minute five times the chamber volume (e.g. if chamber volume is 1 L, we recommend a 5 L/min pump).
• Tubing is required to connect the pumps with the respirometer.
• Replacement tubes are available, please contact us for details.

**35 and 45mm chambers made from glass

Use the respirometers with your own oxygen and data acquisition equipment or with our systems for automatic respirometry.

• Free choice of chamber length, and 8 different inner diameters
• Chambers made for use in full strength sea water
• Easy tool-free handling and cleaning
• Smaller chambers made of glass to avoid O2 storage/release
• Built-in baffles for even distribution of flow

• For flushing and recirculating the water in the chamber pumps are needed. We suggest pumps with a flow rate per minute five times the chamber volume (e.g. if chamber volume is 1 L, we recommend a 5 L/min pump).
• To connect the pumps with the respirometer tubing is needed.
• Place the oxygen probe in one of our inline flow-through chambers (D025, D026, D027).
• Replacement glass tubes are available, please contact us for details.

• Use the respirometers with your own oxygen and data acquisition equipment or with our systems for automatic respirometry.
• Pumps are required for flushing and recirculating water in the chamber. We suggest pumps with a flow rate per minute five times the chamber volume (e.g. if chamber volume is 1 L, we recommend a 5 L/min pump).
• Tubing is required to connect the pumps with the respirometer.
• Replacement tubes are available, please contact us for details.

**35 and 45mm chambers made from glass

Use the respirometers with your own oxygen and data acquisition equipment or with our systems for automatic respirometry.

• Free choice of chamber length, and 8 different inner diameters
• Chambers made for use in full strength sea water
• Easy tool-free handling and cleaning
• Smaller chambers made of glass to avoid O2 storage/release
• Built-in baffles for even distribution of flow

• For flushing and recirculating the water in the chamber pumps are needed. We suggest pumps with a flow rate per minute five times the chamber volume (e.g. if chamber volume is 1 L, we recommend a 5 L/min pump).
• To connect the pumps with the respirometer tubing is needed.
• Place the oxygen probe in one of our inline flow-through chambers (D025, D026, D027).
• Replacement glass tubes are available, please contact us for details.

Features:

• Use the respirometers with your own oxygen and data acquisition equipment or with our systems for automatic respirometry.
• Pumps are required for flushing and recirculating water in the chamber. We suggest pumps with a flow rate per minute five times the chamber volume (e.g. if chamber volume is 1 L, we recommend a 5 L/min pump).
• Tubing is required to connect the pumps with the respirometer.
• Replacement tubes are available, please contact us for details.

• Free choice of chamber length, and 8 different inner diameters
• Chambers made for use in full strength sea water
• Easy tool-free handling and cleaning
• Smaller chambers made of glass to avoid O2 storage/release
• Built-in baffles for even distribution of flow

**35 and 45mm chambers made from glass

Included:

• Glass or acrylic chamber of user-specified length
• Two end caps
• Stainless wing nuts and washers
• Four threaded plastic hose fittings
• Extra rubber o-ring
• Extra two wing nuts and washers

Features:

• Use the respirometers with your own oxygen and data acquisition equipment or with our systems for automatic respirometry.
• Pumps are required for flushing and recirculating water in the chamber. We suggest pumps with a flow rate per minute five times the chamber volume (e.g. if chamber volume is 1 L, we recommend a 5 L/min pump).
• Tubing is required to connect the pumps with the respirometer.
• Replacement tubes are available, please contact us for details.

• Free choice of chamber length, and 8 different inner diameters
• Chambers made for use in full strength sea water
• Easy tool-free handling and cleaning
• Smaller chambers made of glass to avoid O2 storage/release
• Built-in baffles for even distribution of flow

**35 and 45mm chambers made from glass

Included:

• Glass or acrylic chamber of user-specified length
• Two end caps
• Stainless wing nuts and washers
• Four threaded plastic hose fittings
• Extra rubber o-ring
• Extra two wing nuts and washers