The OX1LP Dissolved Oxygen Packages are based on the use of polarographic dissolved oxygen electrodes and may be used to measure photosynthesis and/or respiration in the aqueous phase, using suspensions of plant cells, animals, organelles (chloroplasts and mitochondria) and algae. These packages may also be used to monitor chemical and biochemical reactions that either produce or consume O2 in the aqueous phase. The Dissolved Oxygen Package comes complete with all of the instruments, accessories, software, data acquisition interface and manuals for the instructor and the student.

Description

The OX1LP Dissolved Oxygen Packages are available with DO electrodes of different volumes from 100ul (flow-through) to 50 ml. The O2 electrode within the water-jacketed cuvette may be used for measurements of O2 consumption (or production) by biological samples or enzymatic reactions. Calibration is a simple 2-point process, and all the required accessories for calibration are supplied as part of the package.

The Dissolved Oxygen Package provides excellent accuracy and a resolution of 0.03% O2 (where air saturated water = 100%). Dissolved O2 values may be expressed in any desired units (mg/L; % dissolved O2; ppm etc) based on a simple calibration procedure. The water jacket allows calibrations and experiments to be carried out at the same temperature, or a simple temperature correction can be made in software for measurements made at different temperatures.

Features

Polarographic oxygen electrode system
Different volume cuvette chambers available (1, 2.5, 4, 6, 30, 50ml)
Water-jacketed cuvette allows temperature control with an optional water bath
Includes all the accessories
Easy assembly and calibration

Applications

Algal photosynthesis and respiration
Bacterial respiration
Respiration of small aquatic animals
Aquatic insects and larvae respiration
Mitochondrial respiration
Chloroplast O2 production
Cell suspension respiration
Plant cells suspension photosynthesis
Enzymatic Reactions that consume or produce O2

Components

Dissolved Oxygen Cuvette Electrode (1, 2.5, 4, 6, 30, 50 mL)
A231 Cuvette Electrode Amplifier
A255 Magnetic Stirrer
A135 Stir Bar (2) and O-Ring (2)
A131 KCl Filling Solution
A136 Membrane Material and Filter Paper
10 ft Excelon Tubing for the Water Jacket
C610 LabQuest Mini Data Interface
C901 Logger Pro Software
C404 Customized Setup Software
Instructor’s and Student’s Manuals (include experiments)

Optional Components:

Syringes for addition of metabolites to cuvette; (A141) 10µL, (A143) 50µL, (A145) 100µL
S172 Thermistor for measurements of sample temperature (not available for 1ml cuvette)
A113 calibrated LED light source
Q-Box rugged case for storage and transport of all components

Experiments

Examples of Experiments for OX1LP packages:

Measurement of Aquatic Photosynthetic O2 Evolution
Effect of Inhibitors on Mitochondrial Electron Transport
Analysis of Light-dependent Chloroplast Electron Transport
Effects of Temperature on O2 Uptake by Aquatic Animals
Kinetics of O2-consuming Enzymes

References

Koblizek M et al (2020) Utilization of Light energy in phototropic Gemmatimonadetes. Journal of Photochemistyr and Photobiology B 213: 112085 https://doi.org/10.1016/j.jphotobiol.2020.112085
Nair P, Huertas M, Nowlin WH (2020) Metabolic responses to long-term food deprivation in subterranean and surface amphipods. Subterranean Biology 33: 1-15 https://digital.library.txstate.edu/handle/10877/9805
Henry EF, MacCormack TJ (2018) Taurine protects cardiac contractility in killifish, Fundulus heteroclitus, by enhancing sarcoplasmic reticular Ca²⁺ cycling. Journal of Comparative Physiology B 188:89-99 https://link.springer.com/article/10.1007/s00360-017-1107-4
Lamarre SG et al. (2019) Interrelationship Between Contractility, Protein Synthesis and Metabolism in Mantle of Juvenile Cuttlefish (Sepia officinalis). Frontiers in Physiology V10, 1-14 (doi: 10.3389/fphys.2019.01051)
Sirikhachornkit et al. (2016) Increasing the Triacylglycerol Content in Dunaliella tertiolecta through Isolation of Starch-Deficient Mutants. J. Microbiol. Biotechnol. 26(5), 854–866
Croston, Tara L., et al. (2014) Functional deficiencies of subsarcolemmal mitochondria in the type 2 diabetic human heart. American Journal of Physiology-Heart and Circulatory Physiology 307.1 H54-H65
Sussarellu, Rossana, et al. (2013) Rapid mitochondrial adjustments in response to short-term hypoxia and re-oxygenation in the Pacific oyster, Crassostrea gigas.” The Journal of Experimental Biology 216.9 1561-1569
Dickinson GH et al. (2013) Environmental salinity modulates the effects of elevated CO2 levels on juvenile hard-shell clams, Mercenaria mercenaria. The Journal of experimental biology 216.14 2607-2618.
Bauer I and Kappler A (2009) Rates and Extent of Reduction of Fe(III) Compounds and O₂ by Humic Substances. Environ. Sci. Technol. Vol 43, Number 13, p4902–4908
Mosher CM et al. (2008) Functional Analysis of Phenylalanine Residues in the Active Site of Cytochrome P450 2C9. Biochemistry Vol 47, Number 45, p11725–11734
Mendelsohn BA et al. (2008).The zebrafish embryo as a dynamic model of anoxia tolerance. Developmental Dynamics Vol 237, Issue 7, p1780–1788
Mendelsohn BA and Gitlin JD (2008) Coordination of development and metabolism in the pre-midblastula transition zebrafish embryo. Developmental Dynamics Vol 237, Issue 7, p1789–1798.
Johnson EA, Rosenberg J, McCarty RE. (2007) Expression by Chlamydomonas reinhardtii of a chloroplast ATP synthease with polyhistidine-tagged beta subunits. Biochimica et Biophysica Acta 1767:374-380
Johnson E A. (2008) Altered expression of the chloroplasts ATP synthase through site-directed mutagenesis inChlamydamonas Reinhardtii. Photosynth Res vol 96:153-162
Locuson CW, Gannett PM and Tracy TS (2006) Heteroactivator effects on the coupling and spin state equilibrium of CYP2C9. Archives of Biochemistry and Biophysics Vol 449, Issues 1-2, p115-129